motor neuron maintenance medium Search Results


93
iXCells Biotechnologies motor neuron culture medium a
Motor Neuron Culture Medium A, supplied by iXCells Biotechnologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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motor neuron culture medium a - by Bioz Stars, 2026-03
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Thermo Fisher dmem high glucose
Dmem High Glucose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Axol Bioscience motor neuron recovery medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Motor Neuron Recovery Medium, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/motor neuron recovery medium/product/Axol Bioscience
Average 86 stars, based on 1 article reviews
motor neuron recovery medium - by Bioz Stars, 2026-03
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90
Thermo Fisher mem non-essential amino acids solution
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Mem Non Essential Amino Acids Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mem non-essential amino acids solution - by Bioz Stars, 2026-03
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Axol Bioscience motor neuron maintenance medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Motor Neuron Maintenance Medium, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/motor neuron maintenance medium/product/Axol Bioscience
Average 86 stars, based on 1 article reviews
motor neuron maintenance medium - by Bioz Stars, 2026-03
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90
Thermo Fisher neurobasal medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Neurobasal Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
neurobasal medium - by Bioz Stars, 2026-03
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90
STEMCELL Technologies Inc retinoic acid ra
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Retinoic Acid Ra, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Millipore motor neuron differentiation medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Motor Neuron Differentiation Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
motor neuron differentiation medium - by Bioz Stars, 2026-03
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90
ScienCell motor neuron maturation medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Motor Neuron Maturation Medium, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/motor neuron maturation medium/product/ScienCell
Average 90 stars, based on 1 article reviews
motor neuron maturation medium - by Bioz Stars, 2026-03
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96
Tocris motor neuron progenitor mnp medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Motor Neuron Progenitor Mnp Medium, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc complete motor neuron maintenance medium
(a) Schematic detailing method of iPSC <t>motor</t> <t>neuron</t> loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth <t>medium,</t> DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.
Complete Motor Neuron Maintenance Medium, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete motor neuron maintenance medium/product/Danaher Inc
Average 86 stars, based on 1 article reviews
complete motor neuron maintenance medium - by Bioz Stars, 2026-03
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Image Search Results


(a) Schematic detailing method of iPSC motor neuron loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth medium, DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.

Journal: bioRxiv

Article Title: Bioengineered model of the human motor unit with physiologically functional neuromuscular junctions

doi: 10.1101/2020.05.04.076778

Figure Lengend Snippet: (a) Schematic detailing method of iPSC motor neuron loading to generate neuromuscular tissues at different stages of myogenic development. (i) MN progenitors are loaded to spheroid microplates and matured for 14 days. (ii) Primary HDMCs are seeded in established type I collagen matrix, loaded into 3D printed mould and cultured to maturity for 2 weeks. Matured MNs are then added within optimised type I collagen hydrogel, seeded around SkM and cultured for a further 2 weeks. MN; Motor neuron, SkM; Skeletal muscle, HDMC; Human derived muscle cells, GM; Growth medium, DM; Differentiation medium, ECM; Extracellular matrix, MM; Motor neuron maintenance medium. (b) Addition of iPSC motor neurons with type I collagen perimysium at loading densities of 2, 4 or 6 spheroids per tissue evidence tetanic force and (c) dose dependent spontaneous twitch profiles compared to skeletal muscle only tissues. (d) Quantification of functional tetanic and (e) spontaneous twitch force output with 2, 4 or 6 motor neuron spheroids. Individual functional data points indicative of n=3 contraction profiles, totalling minimum of n=9 and maximum of n=15 per condition and presented ± standard deviation (SD). **P ≤ 0.01 and ***P ≤ 0.001.

Article Snippet: Following resuscitation, iPSC derived motor neuron progenitors (Axol Bioscience, UK, ax0078) were seeded at 2×10 6 cells/T25 culture flask coated with 1.5mg/mL Corning™ Matrigel™ (Fisher) DMEM solution, in motor neuron recovery medium (RM, Axol) supplemented with 0.1µM all-trans retinoic acid (RA, Sigma) and 10µM Y-27332 ROCK inhibitor (Stem Cell Technologies, removed after 24h).

Techniques: Cell Culture, Derivative Assay, Functional Assay, Standard Deviation